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The electrical sensing results of GFET biosensor. (a) (PBASE: 5 mM; aptamer: 5 µM). (b) GFET transfer curves for Aβ42 detection under identical functionalization. (c) Four regions functionalized with <t>Aβ42</t> <t>and</t> <t>hsa-miR-125b</t> aptamers in 1× PBS, with Aβ42 target added for measuring the average signal response at different concentrations. (d) Four regions functionalized with Aβ42 and hsa-miR-125b aptamers in 1× PBS, with hsa-miR-125b target added for measuring the average signal response at different concentrations. (e) The sensor response as a function 125b concentration, with mean, standard deviation, median, and a linear fitting curve. (f) The sensor response as a function Aβ42 concentration, with mean, standard deviation, median, and a linear fitting curve ( n ≥ 13, n is number of sensors used for each measurement) ( n ≥ 13, n is number of sensors used for each measurement).
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The electrical sensing results of GFET biosensor. (a) (PBASE: 5 mM; aptamer: 5 µM). (b) GFET transfer curves for Aβ42 detection under identical functionalization. (c) Four regions functionalized with <t>Aβ42</t> <t>and</t> <t>hsa-miR-125b</t> aptamers in 1× PBS, with Aβ42 target added for measuring the average signal response at different concentrations. (d) Four regions functionalized with Aβ42 and hsa-miR-125b aptamers in 1× PBS, with hsa-miR-125b target added for measuring the average signal response at different concentrations. (e) The sensor response as a function 125b concentration, with mean, standard deviation, median, and a linear fitting curve. (f) The sensor response as a function Aβ42 concentration, with mean, standard deviation, median, and a linear fitting curve ( n ≥ 13, n is number of sensors used for each measurement) ( n ≥ 13, n is number of sensors used for each measurement).
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The electrical sensing results of GFET biosensor. (a) (PBASE: 5 mM; aptamer: 5 µM). (b) GFET transfer curves for Aβ42 detection under identical functionalization. (c) Four regions functionalized with <t>Aβ42</t> <t>and</t> <t>hsa-miR-125b</t> aptamers in 1× PBS, with Aβ42 target added for measuring the average signal response at different concentrations. (d) Four regions functionalized with Aβ42 and hsa-miR-125b aptamers in 1× PBS, with hsa-miR-125b target added for measuring the average signal response at different concentrations. (e) The sensor response as a function 125b concentration, with mean, standard deviation, median, and a linear fitting curve. (f) The sensor response as a function Aβ42 concentration, with mean, standard deviation, median, and a linear fitting curve ( n ≥ 13, n is number of sensors used for each measurement) ( n ≥ 13, n is number of sensors used for each measurement).
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The electrical sensing results of GFET biosensor. (a) (PBASE: 5 mM; aptamer: 5 µM). (b) GFET transfer curves for Aβ42 detection under identical functionalization. (c) Four regions functionalized with Aβ42 and hsa-miR-125b aptamers in 1× PBS, with Aβ42 target added for measuring the average signal response at different concentrations. (d) Four regions functionalized with Aβ42 and hsa-miR-125b aptamers in 1× PBS, with hsa-miR-125b target added for measuring the average signal response at different concentrations. (e) The sensor response as a function 125b concentration, with mean, standard deviation, median, and a linear fitting curve. (f) The sensor response as a function Aβ42 concentration, with mean, standard deviation, median, and a linear fitting curve ( n ≥ 13, n is number of sensors used for each measurement) ( n ≥ 13, n is number of sensors used for each measurement).

Journal: RSC Advances

Article Title: Graphene field-effect transistor based multiplexed sensing platform for simultaneous detection of multiple Alzheimer's disease biomarkers

doi: 10.1039/d5ra07384g

Figure Lengend Snippet: The electrical sensing results of GFET biosensor. (a) (PBASE: 5 mM; aptamer: 5 µM). (b) GFET transfer curves for Aβ42 detection under identical functionalization. (c) Four regions functionalized with Aβ42 and hsa-miR-125b aptamers in 1× PBS, with Aβ42 target added for measuring the average signal response at different concentrations. (d) Four regions functionalized with Aβ42 and hsa-miR-125b aptamers in 1× PBS, with hsa-miR-125b target added for measuring the average signal response at different concentrations. (e) The sensor response as a function 125b concentration, with mean, standard deviation, median, and a linear fitting curve. (f) The sensor response as a function Aβ42 concentration, with mean, standard deviation, median, and a linear fitting curve ( n ≥ 13, n is number of sensors used for each measurement) ( n ≥ 13, n is number of sensors used for each measurement).

Article Snippet: Aβ42 and Aβ40 peptides, hsa-miR-125b RNA sequence, the hsa-miR-125b complementary DNA probe and the Aβ42 DNA aptamer were synthesized by Sangon Biotech (Shanghai) Co., Ltd.

Techniques: Concentration Assay, Standard Deviation

Mixed sample detection results. (a) Higher concentration of Aβ42 biomarker vs. lower concentration of hsa-miR-125b biomarker. The absolute concentrations were (Aβ42 : 125b): 10 pM : 1 fM (10 000 : 1); 50 nM : 50 pM (1000 : 1); and 100 nM : 1 nM (100 : 1). (b) Higher concentration of hsa-miR-125b biomarker vs. lower concentration of Aβ42 biomarker. The absolute concentrations were (125b : Aβ42): 100 nM : 1 fM (10 8 : 1), 100 nM : 1 pM (10 6 : 1), and 50 nM : 50 pM (10 3 : 1). (c) Fixed concentration of Aβ40 biomarker and varying concentrations of hsa-miR-125b ( n ≥ 13, n is number of sensors used for each measurement).

Journal: RSC Advances

Article Title: Graphene field-effect transistor based multiplexed sensing platform for simultaneous detection of multiple Alzheimer's disease biomarkers

doi: 10.1039/d5ra07384g

Figure Lengend Snippet: Mixed sample detection results. (a) Higher concentration of Aβ42 biomarker vs. lower concentration of hsa-miR-125b biomarker. The absolute concentrations were (Aβ42 : 125b): 10 pM : 1 fM (10 000 : 1); 50 nM : 50 pM (1000 : 1); and 100 nM : 1 nM (100 : 1). (b) Higher concentration of hsa-miR-125b biomarker vs. lower concentration of Aβ42 biomarker. The absolute concentrations were (125b : Aβ42): 100 nM : 1 fM (10 8 : 1), 100 nM : 1 pM (10 6 : 1), and 50 nM : 50 pM (10 3 : 1). (c) Fixed concentration of Aβ40 biomarker and varying concentrations of hsa-miR-125b ( n ≥ 13, n is number of sensors used for each measurement).

Article Snippet: Aβ42 and Aβ40 peptides, hsa-miR-125b RNA sequence, the hsa-miR-125b complementary DNA probe and the Aβ42 DNA aptamer were synthesized by Sangon Biotech (Shanghai) Co., Ltd.

Techniques: Concentration Assay, Biomarker Discovery